Plasmid

Part:BBa_K5173000:Design

Designed by: Jiayi Chen   Group: iGEM24_EHSBNU   (2024-09-30)


pET-28a plasmid with optimized tachypleus tridentatus clotting factor G alpha subunit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We downloaded and optimized the mature peptide sequence so that the eukaryote-derived protein may be suitable for expression in a prokaryotic expression system.


Source

We optimized the sequence of tachypleus tridentatus clotting factor G alpha subunit, an eukaryotic protein, downloaded from GenBank, for more efficient protein expression in prokaryotic e.coli BL21 cells.We then inserted the optimized sequence into the multiple cloning site of pET-28a plasmid, which we downloaded from SnapGene.

References